Umkhuhlane A/B + RSV Antigen Rapid Test Kit (I-Nasal Swab Test)

Umkhuhlane yisifo esithathelwanayo kakhulu, esiyingozi, esibangelwa amagciwane emgudwini wokuphefumula.Ama-causative agents alesi sifo ahlukene ngokwe-immunologically, amagciwane e-RNA anomucu owodwa aziwa ngokuthi amagciwane omkhuhlane.Kunezinhlobo ezintathu zamagciwane omkhuhlane: A, B, kanye no-C. Amagciwane ohlobo A avame kakhulu futhi ahlotshaniswa nezifo eziwumshayabhuqe ezimbi kakhulu.Amagciwane oHlobo B akhiqiza isifo ngokuvamile esithambile kuneso esibangelwa uhlobo A. Amagciwane ohlobo C awakaze ahlotshaniswe nobhadane olukhulu lwesifo somuntu.Womabili amagciwane ohlobo A no-B angajikeleza ngesikhathi esisodwa, kodwa ngokuvamile uhlobo olulodwa lunamandla phakathi nesizini ethile.Ama-antigens omkhuhlane angase atholwe kumasampula omtholampilo nge-immunoassay.Ukuhlolwa Komkhuhlane A+B ukuhlolwa kwe-lateral-flow immunoassay esebenzisa amasosha omzimba azwela kakhulu e-monoclonal aqondene ngqo nama-antigen omkhuhlane.Ukuhlolwa kuqondiswe ezinhlotsheni zomkhuhlane A no-B angakwazi ukuhlangana kabusha kwezitshalo ezivamile noma amanye amagciwane aziwayo okuphefumula.

I-Respiratory Syncytial Virus(RSV) iyimbangela evame kakhulu ye-bronchiolitis kanye nenyumoniya phakathi kwezinsana nezingane ezineminyaka engaphansi ko-1 ubudala. I-III iqala kakhulu ngemfiva, ikhala eligelezayo, ukukhwehlela kanye nokuhefuzela ngezinye izikhathi.Isifo esibi kakhulu sokuphefumula okuphansi singenzeka kunoma ibuphi ubudala, ikakhulukazi kubantu asebekhulile noma kulabo abanamasosha omzimba, amaphaphu noma amasosha omzimba asengozini.

uketshezi lokuphefumula ngokuthintana eduze nabantu abanaleli gciwane noma ukuthintana nezinto ezingcolile noma izinto.

Ikhithi Yokuhlola I-A/B+RSV Antigen Rapid Test isekelwe kumgomo wekhwalithi ye-immunochromatographic assay ukuze kutholwe ama-antigens oMkhuhlane A/B+RSV esibonelweni se-Nasal Sawb.I-StripA iqukethe: Amasosha omzimba alwa nomkhuhlane A no-B ingakwazi ukuhamba endaweni yokuhlola A no-B yolwelwesi ngokulandelana.Ngesikhathi sokuhlolwa, isifanekiso esikhishiwe sibhekana nama-anti-influenza A no-B amasosha omzimba ahlanganiswe ezinhlayiyeni ezinemibala futhi ahlanganiswe ngaphambili kuphedi yesampula yokuhlolwa.Ingxube ibe isihamba nge-membrane ngesenzo se-capillary futhi ixhumana nama-reagents kulwelwesi.Uma kukhona ama-antigens egciwane lomkhuhlane A no-B owanele kusifanekiso, amabhendi anombala azokwakheka endaweni yokuhlola yolwelwesi.I-strip B iqukethe : 1) i-burgundy colored conjugate pad equkethe i-antigen recombinant ehlanganiswe negolide le-colloid (igundane le-monoclonal anti-Respiratory Syncytial Virus(RSV) antibody conjugates) kanye nezihlanganisi zonogwaja we-IgG-gold, 2) i-nitrocelland stripT equkethe ulwelwesi lwe-nitrocellulose amabhendi) kanye nebhendi yokulawula (ibhendi C).I-T band imbozwe ngaphambili nge-monoclonal mouse anti-Respiratory Syncytial Virus(RSV) antibody ukuze kutholwe i-Respiratory Syncytial Virus(RSV) glycoprotein F antigen, futhi ibhande C limbozwe ngaphambili nge-anti-rabbit IgG yembuzi.

I-Strip A: Ingxube ibe isihamba nge-membrane ngesenzo se-capillary futhi ixhumana nama-reagents kulwelwesi.Uma kukhona ama-antigens egciwane lomkhuhlane A no-B owanele kusifanekiso, amabhendi anombala azokwakheka endaweni yokuhlola yolwelwesi.Ukuba khona kwebhande elinemibala esifundeni A kanye/noma B kubonisa umphumela omuhle kuma-antigen athile egciwane, kuyilapho ukungabikho kwawo kubonisa umphumela omubi.Ukubukeka kwebhande elinemibala endaweni yokulawula kusebenza njengokulawula kwenqubo, okubonisa ukuthi umthamo ofanele we-specimen ungeziwe futhi i-membrane wicking yenzekile.

I-strip B:Lapho ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.I-Respiratory Syncytial Virus(RSV) uma ikhona kusifanekiso izobophezela kuma-monoclonal mouse anti-Respiratory Syncytial Virus(RSV) antibody conjugates.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-anti-Respiratory Syncytial Virus(RSV) yegundane eboshwe ngaphambili, yakhe ibhendi ye-T enombala obomvana, okubonisa umphumela wokuhlolwa oqondile we-Respiratory Syncytial Virus(RSV).Ukungabikho kwebhendi yokuhlola (T) kuphakamisa umphumela ongemuhle.Ukuhlolwa kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinemibala e-burgundy ye-immunocomplex ye-goat anti rabbit IgG/rabbit IgG-gold conjugate kungakhathaliseki ukuthuthuka kombala kunoma yimaphi amabhendi okuhlola.Ngaphandle kwalokho