ISIFINYEZO KANYE NENCAZELO ISIVIVINYO
I-Leptospirosis yenzeka emhlabeni wonke futhi iyinkinga evamile yezempilo emaphakathi kuya kwenzima kubantu nasezilwaneni, ikakhulukazi ezindaweni ezinesimo sezulu esishisayo nesinomswakama.Izizinda zemvelo ze-leptospirosis amagundane kanye nezinhlobonhlobo ezinkulu zezilwane ezincelisayo ezifuywayo.Ukutheleleka kwabantu kubangelwa ama-L. interrogans, ilungu le-pathogenic lohlobo lwe-Leptospira.Ukutheleleka kusakazwa ngomchamo ovela esilwaneni esisingethe.
Ngemuva kokutheleleka, ama-leptospires akhona egazini aze asuswe ngemva kwezinsuku ezi-4 kuya kweziyi-7 ngemva kokukhiqizwa kwe-anti-L.interrogans amasosha omzimba, ekuqaleni kwesigaba IgM.Isiko legazi, umchamo kanye noketshezi lwe-cerebrospinal kuyindlela ephumelelayo yokuqinisekisa ukuxilongwa phakathi neviki lokuqala kuya kwelesi-2 ngemva kokuchayeka.Ukutholwa kwe-serological ye-antiL.I-interrogans antibodies iyindlela evamile yokuxilonga.Ukuhlolwa kuyatholakala ngaphansi kwalesi sigaba: 1) Ukuhlolwa kwe-microscopic agglutination (MAT);2) ELISA;3) Ukuhlolwa kwe-antibody ye-fluorescent engaqondile (IFATs).Kodwa-ke, zonke izindlela ezibalulwe ngenhla zidinga indawo eyinkimbinkimbi kanye nochwepheshe abaqeqeshwe kahle.
I-Leptospira IgG/IgM isivivinyo se-serological esilula esisebenzisa ama-antigens avela ku-L. interrogans futhi ithola amasosha omzimba e-IgG ne-IgM kulawa ma-microorganisms kanyekanye.Ukuhlolwa kungenziwa izisebenzi ezingaqeqeshiwe noma ezinamakhono amancane, ngaphandle kwemishini yaselabhorethri enzima futhi umphumela utholakala phakathi nemizuzu eyi-15.
ISIMISO
I-Leptospira IgG/IgM Rapid Test Kit iyi-chromatographic egeleza yangemuva.
ukuhlolwa kwe-immunoassay.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe i-recombinant L. interrogans antigens ehlanganiswe negolide eli-colloid (ama-Leptospira conjugates) nama-conjugate kanogwaja we-IgG-gold, 2) ulwelwesi lwe-nitrocellulose oluqukethe amabhendi amabili okuhlola (amabhendi angu-M no-G) kanye nebhendi yokulawula (ibhendi C).Ibhendi engu-M imbozwe ngaphambilini nge-monoclonal anti-human IgM ukuze kutholwe i-anti-L.. i-interrogans IgM, G band imbozwe ngaphambili ngama-reagents ukuze kutholwe i-anti-L.i-interrogans IgG, kanye nebhendi ye-C imbozwe ngaphambili nge-anti rabbit IgG.
Uma ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.I-IgM anti-L.. ama-interrogans uma ekhona kusifanekiso azobophezela kuma-conjugates e-Leptospira.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-anti-human IgM antibody, eyenza ibhande elingu-burgundy elingu-M, elibonisa umphumela wokuhlola we-L.. interrogans IgM.I-IgG anti-L.. ama-interrogans uma ekhona kusifanekiso azobophezela kuma-conjugates e-Leptospira.I-immunocomplex bese ibanjwa ama-reagents ahlanganiswe ngaphambili kulwelwesi, enze ibhande le-G elinemibala e-burgundy, ekhombisa umphumela wokuhlola we-L.. interrogans IgG.
Ukungabikho kwanoma yimaphi amabhendi okuhlola (M no-G) kuphakamisa umphumela ongemuhle.Ukuhlolwa kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinemibala e-burgundy ye-immunocomplex ye-goat anti rabbit IgG/rabbit IgG-gold conjugate kungakhathaliseki ukuthuthuka kombala kunoma yimaphi amabhendi okuhlola.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.
