ISIFINYEZO KANYE NENCAZELO ISIVIVINYO
Umalaleveva yisifo esithwalwa umiyane, i-hemolytic, i-febrile esihlasela abantu abangaphezu kwezigidi ezingama-200 futhi sibulala abantu abangaphezu kwesigidi esisodwa ngonyaka.Ibangelwa izinhlobo ezine zePlasmodium: P. falciparum, P. vivax, P. ovale, kanye ne-P. malariae.Le plasmodia yonke ingena futhi ibulale ama-erythrocyte abantu, ikhiqize ukugodola, imfiva, i-anemia, ne-splenomegaly.I-P. falciparum idala isifo esiyingozi kakhulu kunezinye izinhlobo ze-plasmodial futhi ibangela ukufa kwabantu abaningi umalaleveva.I-P. falciparum kanye ne-P. vivax yizifo ezivame kakhulu, nokho, kunokwehluka okukhulu kwendawo ekusabalaleni kwezinhlobo zezilwane.
Ngokwesiko, umalaleveva utholwa ngokuboniswa kwezinto eziphilayo ku-Giemsa ama-smear aminyene anegazi le-peripheral, futhi izinhlobo ezahlukene ze-plasmodium zihlukaniswa ngokubonakala kwazo kuma-erythrocyte angenwe yilesi sifo.Inqubo iyakwazi ukuxilonga okunembayo nokuthembekile, kodwa kuphela uma kwenziwa osolwazi abanekhono besebenzisa izivumelwano ezichaziwe, ezethula izithiyo ezinkulu ezindaweni ezikude nezimpofu zomhlaba.
I-Malaria Pf/Pv Ag Rapid Test yenzelwe ukuxazulula lezi zithiyo.Isebenzisa amasosha omzimba aqondene ne-P. falciparum Histidine Rich Protein-II (pHRP-II) kanye ne-P. vivax Lactate Dehydrogenase (Pv-LDH) ukuze ibone ngesikhathi esisodwa futhi ihlukanise ukutheleleka nge-P. falciparum ne-P. vivax.Ukuhlolwa kungenziwa ngabasebenzi abangaqeqeshiwe noma abanamakhono amancane, ngaphandle kwemishini yaselabhorethri
ISIMISO
I-Malaria Pf/Pv Ag Rapid Test iyi-lateral flow chromatographic immunoassay.Izingxenye zokuhlola ze-strip zihlanganisa: 1) i-burgundy colored conjugate pad equkethe i-anti-Pv-LDH antibody yegundane ehlanganiswe negolide eliyi-colloid (Pv-LDH-gold conjugates) kanye ne-mouse anti-pHRP-II antibody ehlanganiswe negolide eliyi-colloid (pHRP-II -i-conjugates yegolide), 2) i-nitrocellulose membrane strip equkethe amabhendi amabili okuhlola (amabhendi e-Pv ne-Pf) kanye nebhendi yokulawula (i-C band).Ibhendi ye-Pv ihlanganiswe ngaphambili nelinye igundane elivikela i-Pv-LDH elikhethekile ukuze kutholwe ukutheleleka kwe-Pv, ibhendi ye-Pf ifakwe ngaphambili ngamasosha omzimba e-polyclonal anti-pHRP-II ukuze kutholwe ukutheleleka kwe-Pf, futhi ibhande C limbozwe. ngembuzi anti-mouse IgG.
Ngesikhathi sokuhlolwa, ivolumu eyanele yesampula yegazi ikhishelwa emthonjeni wesampula (S) wekhasethi lokuhlola, i-lysis buffer yengezwa kumthombo we-buffer (B).Isigcinalwazi siqukethe okokuhlanza okulalisa amangqamuzana egazi abomvu futhi kukhiphe ama-antigen ahlukahlukene, ahamba ngesenzo se-capillary emgqeni ogcinwe kukhasethi.I-Pv-LDH uma iphrezentheshini kusifanekiso izobophezela kuma-conjugates e-Pv-LDH-gold.I-immunocomplex ibe isithwetshulwa kulwelwesi yi-anti-Pv-LDH ehlanganiswe ngaphambili, yakha ibhendi ye-Pv enombala obomvana, ekhombisa umphumela wokuhlola we-Pv.Kungenjalo, i-pHRP-II uma iphrezentheshini kusifanekiso izobophezela ku-pHRP-II-conjugates yegolide.I-immunocomplex ibe isithwetshulwa kulwelwesi ngamasosha omzimba ahlanganiswe ngaphambili e-anti-pHRP-II, enza ibhande le-Pf elinemibala e-burgundy, ekhombisa umphumela wokuhlola we-Pf.Ukungabikho kwanoma yimaphi amabhendi okuhlola kuphakamisa umphumela ongemuhle.Ukuhlolwa kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala obomvana le-immunocomplex yembuzi elwa negundane IgG / igundane IgG (anti-Pv-LDH kanye ne-anti-pHRPII) -conjugates yegolide kungakhathalekile ukuthuthuka kombala kunoma iyiphi yamabhendi okuhlola.Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.
