I-Qrf Antibody Test Ishidi Elinganqunyiwe

Ukuhlolwa kwe-Qrf Antibody

Uhlobo: Ishidi elingasikiwe

Igama Brand: I-Bio-mapper

Ikhathalogi: REA0911

Isampula: WB/S/P

Ikhithi yokuhlola igciwane lengculazi yezimvu isetshenziselwa ukubona amasosha omzimba wemvukuzane kuseramu yezimvu, engasetshenziswa ukuhlola umphumela wokuzivikela komzimba womuthi wokugomela upokisi wezimvu noma ukusiza ekuhlonzweni kwesifo.Umgomo ukuthi ikhithi yokutholwa kwegciwane le-imvu pox virus yakhiwe ngepuleti le-antigen le-protein le-nyukliya eliboshwe ngaphambili, izimpawu ze-enzyme nezinye izinto ezisekelayo, futhi isimiso se-enzyme-linked immunoassay (ELISA) sisetshenziswa ukuze kutholakale upokisi wezimvu. amasosha omzimba kusampula serum yezimvu.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo enemininingwane

Ukutholwa kwe-antibody ye-imvu pox virus kwakhiwa i-microplate pre-coated ne-imvu pox nuclear protein antigen, izimpawu ze-enzyme nezinye izinto ezisekelayo, futhi isimiso se-enzyme-linked immunoassay (ELISA) sisetshenziselwa ukuthola i-antibody pox yezimvu isampula ye-serum yezimvu.Ngesikhathi sokuhlolwa, i-serum yokulawula kanye nesampula okufanele ihlolwe kwengezwa epuletini le-microplate, futhi uma isampula iqukethe i-antibody pox yezimvu ngemva kokufukamela, izoboshelwa ku-antigen ku-microplate plate, nezinye izakhi ezingaboshiwe. izokhishwa ngemva kokugeza;Bese wengeza umaka we-enzayimu ukuze ubophezele ngokuqondile ku-antigen-antibody complex ku-microplate plate;Izimpawu ze-enzyme ezingaboshiwe zabe sezisuswa ngokuwashwa, futhi isisombululo se-TMB substrate sengezwa emithonjeni, futhi umkhiqizo oluhlaza okwesibhakabhaka wakhiwa ukusabela kwe-microplate conjugates, futhi ukujula kombala kwakuhlotshaniswa kahle nenani elithile lama-antibodies aqukethwe ku-microplate conjugates. isampula.Ngemuva kokuthi isisombululo sokuqeda sengezwe ukuze kuqedwe ukusabela, umkhiqizo waphenduka ophuzi;Inani lokumunca ekuphenduleni ngakunye linqunywa isifundi se-microplate kubude begagasi obungu-450 nm ukuze kutholwe ukuthi ingabe isampuli iqukethe amasosha omzimba wepokisi yezimvu.